Preventing, punishing and eradicating gender-based violence, as well as incorporating a gender-based approach in the design of policies, have become the main focus of global debates in recent years, as an integral part of human rights, sustainable social development and democracies. International declarations such as that of the United Nations in 1993 to eliminate this violence, particularly against women, indicate an emerging global consensus on the nature of the problem (Van der Vleuten, et al, 2020) as well as the international conferences and regional networks of activists formed during those years. Although intergovernmental organizations are not intrinsically sensitive to gender, from the 1990s onwards they began to pay greater attention to it, largely due to the influence of the women’s and feminist movement that influenced these spaces. In Latin America and the Caribbean, these issues have a stronger resonance due to the fact that it registers one of the highest rates of gender-based violence and inequality compared to other regions of the world (UNDP, 2019; ECLAC, 2018). The OAS and MERCOSUR are no strangers to these processes and are part of the complex regional framework. This study argues that both organizations contribute to regional gender governance, because they have managed to build useful tools for the promotion and protection of rules that seek to eradicate violence and promote gender equality and equity. The contributions made by these two organizations between 1990 and 2015 are identified and analyzed.